Ca²⁺ regulation of glutamate release from inner hair cells of hearing mice.

ABSTRACT: In our hearing organ, sound is encoded at ribbon synapses formed by inner hair cells (IHCs) and spiral ganglion neurons (SGNs). How the underlying synaptic vesicle (SV) release is controlled by Ca²⁺ in IHCs of hearing animals remained to be investigated. Here, we performed patch-clamp SGN recordings of the initial rate of release evoked by brief IHC Ca²⁺-influx in an ex vivo cochlear preparation from hearing mice. We aimed to closely mimic physiological conditions by perforated-patch recordings from IHCs kept at the physiological resting potential and at body temperature. We found release to relate supralinearly to Ca²⁺-influx (power, m: 4.3) when manipulating the [Ca²⁺] available for SV release by Zn²⁺-flicker-blocking of the s

SUBMITTER: Jaime Tobon LM 

PROVIDER: S-EPMC10710057 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature