Structure of the <i>Lysinibacillus sphaericus</i> Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX.

ABSTRACT: The Lysinibacillus sphaericus proteins Tpp49Aa1 and Cry48Aa1 can together act as a toxin toward the mosquito Culex quinquefasciatus and have potential use in biocontrol. Given that proteins with sequence homology to the individual proteins can have activity alone against other insect species, the structure of Tpp49Aa1 was solved in order to understand this protein more fully and inform the design of improved biopesticides. Tpp49Aa1 is naturally expressed as a crystalline inclusion within the host bacterium, and MHz serial femtosecond crystallography using the novel nanofocus option at an X-ray free electron laser allowed rapid and high-quality data collection to determine the structure of Tpp49Aa1 at 1.62 Å resolution. This revealed the packing of Tpp49Aa1 within these natura

SUBMITTER: Williamson LJ 

PROVIDER: S-EPMC10710082 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature