Genomic DNA extraction optimization and validation for genome sequencing using the marine gastropod Kellet's whelk.

ABSTRACT: Next-generation sequencing technologies, such as Nanopore MinION, Illumina Hiseq and Novaseq, and PacBio Sequel II, hold immense potential for advancing genomic research on non-model organisms, including the vast majority of marine species. However, application of these technologies to marine invertebrate species is often impeded by challenges in extracting and purifying their genomic DNA due to high polysaccharide content and other secondary metabolites. In this study, we help resolve this issue by developing and testing DNA extraction protocols for Kellet's whelk (Kelletia kelletii), a subtidal gastropod with ecological and commercial importance, by comparing four DNA extraction methods commonly used in marine invertebrate studies. In our comparison of extraction methods, the Salt

SUBMITTER: Daniels BN 

PROVIDER: S-EPMC10710129 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature