D variants at the RhD vestibule in the weak D type 4 and Eurasian D clusters.

ABSTRACT:

Background

One branch of the RHD phylogenetic tree is represented by the weak D type 4 cluster of alleles with F223V as the primordial amino acid substitution. F223V as well as a large number of further substitutions causing D variants are located at the extracellular RhD protein vestibule, which represents the entrance to the transmembraneous channel of the RhD protein.

Study design and methods

RHD and RHCE nucleotide sequences were determined from genomic DNA and cDNA. D epitope patterns were established with commercial monoclonal anti-D panels.

Results

The RHD alleles DOL-1 and DOL-2 had the two amino acid substitutions M170T (509T>C) and F223V (667T>G) in common. DOL-2 harbored the additional substitution L378V (1132C>G). Both alleles were observed in Africans and

SUBMITTER: Flegel WA 

PROVIDER: S-EPMC10710224 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature