Single-cell map of dynamic cellular microenvironment of radiation-induced intestinal injury.

ABSTRACT: Intestine is a highly radiation-sensitive organ that could be injured during the radiotherapy for pelvic, abdominal, and retroperitoneal tumors. However, the dynamic change of the intestinal microenvironment related to radiation-induced intestine injury (RIII) is still unclear. Using single-cell RNA sequencing, we pictured a dynamic landscape of the intestinal microenvironment during RIII and regeneration. We showed that the various cell types of intestine exhibited heterogeneous radiosensitivities. We revealed the distinct dynamic patterns of three subtypes of intestinal stem cells (ISCs), and the cellular trajectory analysis suggested a complex interconversion pattern among them. For the immune cells, we found that Ly6c⁺ monocytes can give rise to both pro-inflammatory macroph

SUBMITTER: Lu H 

PROVIDER: S-EPMC10710489 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature