Comprehensive modeling of cell culture profile using Raman spectroscopy and machine learning.

ABSTRACT: Chinese hamster ovary (CHO) cells are widely utilized in the production of antibody drugs. To ensure the production of large quantities of antibodies that meet the required specifications, it is crucial to monitor and control the levels of metabolites comprehensively during CHO cell culture. In recent years, continuous analysis methods employing on-line/in-line techniques using Raman spectroscopy have attracted attention. While these analytical methods can nondestructively monitor culture data, constructing a highly accurate measurement model for numerous components is time-consuming, making it challenging to implement in the rapid research and development of pharmaceutical manufacturing processes. In this study, we developed a comprehensive, simple, and automated method for constructing a

SUBMITTER: Tanemura H 

PROVIDER: S-EPMC10710501 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature