The distinct translational landscapes of gram-negative Salmonella and gram-positive Listeria.

ABSTRACT: Translational control in pathogenic bacteria is fundamental to gene expression and affects virulence and other infection phenotypes. We used an enhanced ribosome profiling protocol coupled with parallel transcriptomics to capture accurately the global translatome of two evolutionarily distant pathogenic bacteria-the Gram-negative bacterium Salmonella and the Gram-positive bacterium Listeria. We find that the two bacteria use different mechanisms to translationally regulate protein synthesis. In Salmonella, in addition to the expected correlation between translational efficiency and cis-regulatory features such as Shine-Dalgarno (SD) strength and RNA secondary structure around the initiation codon, our data reveal an effect of the 2^nd and 3^rd codons, where the presence

SUBMITTER: Bryant OJ 

PROVIDER: S-EPMC10710512 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature