Efficient de novo production of bioactive cordycepin by Aspergillus oryzae using a food-grade expression platform.

ABSTRACT:

Background

Cordycepin (3'-deoxyadenosine) is an important bioactive compound in medical and healthcare markets. The drawbacks of commercial cordycepin production using Cordyceps spp. include long cultivation periods and low cordycepin yields. To overcome these limitations and meet the increasing market demand, the efficient production of cordycepin by the GRAS-status Aspergillus oryzae strain using a synthetic biology approach was developed in this study.

Results

An engineered strain of A. oryzae capable of cordycepin production was successfully constructed by overexpressing two metabolic genes (cns1 and cns2) involved in cordycepin biosynthesis under the control of constitutive promoters. Investigation of the flexibility of carbon utilization for cordycepin production by th

SUBMITTER: Jeennor S 

PROVIDER: S-EPMC10710699 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature