Spinal apolipoprotein E is involved in inflammatory pain via regulating lipid metabolism and glial activation in the spinal dorsal horn.

ABSTRACT:

Introduction

Inflammation and nerve injury promote astrocyte activation, which regulates the development and resolution of pain, in the spinal dorsal horn. APOE regulates lipid metabolism and is predominantly expressed in the astrocytes. However, the effect of astrocytic APOE and lipid metabolism on spinal cellular function is unclear. This study aimed to investigate the effect of spinal Apoe on spinal cellular functions using the complete Freund's adjuvant (CFA)-induced inflammatory pain mouse model.

Methods

After intraplantar injection of CFA, we assessed pain behaviors in C57BL6 and Apoe knockout (Apoe^-/-) mice using von Frey and Hargreaves' tests and analyzed dorsal horn samples (L4-5) using western blotting, immunofluorescence, quantitative real-time polymera

SUBMITTER: Liu S 

PROVIDER: S-EPMC10710718 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature