A single shell protein plays a major role in choline transport across the shell of the choline utilization microcompartment of Escherichia coli 536.

ABSTRACT: Bacterial microcompartments (MCPs) are widespread protein-based organelles that play important roles in the global carbon cycle and in the physiology of diverse bacteria, including a number of pathogens. MCPs consist of metabolic enzymes encapsulated within a protein shell. The main roles of MCPs are to concentrate enzymes together with their substrates (to increase reaction rates) and to sequester harmful metabolic intermediates. Prior studies indicate that MCPs have a selectively permeable protein shell, but the mechanisms that allow selective transport across the shell are not fully understood. Here we examine transport across the shell of the choline utilization (Cut) MCP of Escherichia coli 536, which has not been studied before. The shell of the Cut MCP is unusual in consistin

SUBMITTER: Ochoa JM 

PROVIDER: S-EPMC10710832 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature