Downregulation of MALAT1 in triple-negative breast cancer cells.

ABSTRACT:

Background

MALAT1 is one of the most abundant nuclear long non-coding RNAs, which has been found to be elevated in various types of cancers. However, conflicting reports on MALAT1 in breast cancer cell lines challenge understanding of MALAT1's involvement in breast cancer progression.

Aim

Measurement of normalized relative quantity (NRQ) of MALAT1 transcripts in cell lines representing triple-negative breast cancer (TNBC) and luminal breast cancer.

Materials and methods

The studies were performed using cell lines representing luminal breast cancer (T47D, MCF-7), TNBC (MDA-MB-468, CAL-51, MDA-MB-231), and MCF-10A cell line of normal breast epithelial cells. Total RNA was isolated from six independent cell cultures of each line, treated with DNase I, and used to

SUBMITTER: Klopotowska D 

PROVIDER: S-EPMC10711027 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature