Polymorphisms and expression levels of TNP2, SYCP3, and AZFa genes in patients with azoospermia.

ABSTRACT:

Objective

Azoospermia (the total absence of sperm in the ejaculate) affects approximately 10% of infertile males. Despite diagnostic advances, azoospermia remains the most challenging issue associated with infertility treatment. Our study evaluated transition nuclear protein 2 (TNP2) and synaptonemal complex protein 3 (SYCP3) polymorphisms, azoospermia factor a (AZFa) microdeletion, and gene expression levels in 100 patients with azoospermia.

Methods

We investigated a TNP2 single-nucleotide polymorphism through polymerase chain reaction (PCR) restriction fragment length polymorphism analysis using a particular endonuclease. An allele-specific PCR assay for SYCP3 was performed utilizing two forward primers and a common reverse primer in two PCR reactions. Based on the Europea

SUBMITTER: Jebur MII 

PROVIDER: S-EPMC10711250 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature