The intra-host evolutionary landscape and pathoadaptation of persistent <i>Staphylococcus aureus</i> in chronic rhinosinusitis.

ABSTRACT: Chronic rhinosinusitis (CRS) is a common chronic sinonasal mucosal inflammation associated with Staphylococcus aureus biofilm and relapsing infections. This study aimed to determine rates of S. aureus persistence and pathoadaptation in CRS patients by investigating the genomic relatedness and antibiotic resistance/tolerance in longitudinally collected S. aureus clinical isolates. A total of 68 S. aureus paired isolates (34 pairs) were sourced from 34 CRS patients at least 6 months apart. Isolates were grown into 48 h biofilms and tested for tolerance to antibiotics. A hybrid sequencing strategy was used to obtain high-quality reference-grade assemblies of all isolates. Single nucleotide variants (SNV) divergence in the core genome and sequence type cluste

SUBMITTER: Houtak G 

PROVIDER: S-EPMC10711304 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature