Gene delivery to breast cancer by incorporated EpCAM targeted DARPins into AAV2.

ABSTRACT:

Objective

The aim of this study is to evaluate an AAV vector that can selectively target breast cancer cells and to investigate its specificity and anti-tumor effects on breast cancer cells both in vitro and in vivo, offering a new therapeutic approach for the treatment of EpCAM-positive breast cancer.

Methods

In this study, a modified AAV2 viral vector was used, in which EpCAM-specific DARPin EC1 was fused to the VP2 protein of AAV2, creating a viral vector that can target breast cancer cells. The targeting ability and anti-tumor effects of this viral vector were evaluated through in vitro and in vivo experiments.

Results

The experimental results showed that the AAV2M^EC1 virus could specifically infect EpCAM-positive breast cancer cells and accurately deli

SUBMITTER: Lv YF 

PROVIDER: S-EPMC10712102 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature