In vivo safety and biodistribution profile of Klotho-enhanced human urine-derived stem cells for clinical application.

ABSTRACT:

Background

Urine-derived stem cells (UDSCs) can be easily isolated from urine and possess excellent stem cell characteristics, making them a promising source for cell therapeutics. Due to their kidney origin specificity, UDSCs are considered a superior therapeutic alternative for kidney diseases compared to other stem cells. To enhance the therapeutic potential of UDSCs, we developed a culture method that effectively boosts the expression of Klotho, a kidney-protective therapeutic factor. We also optimized the Good Manufacturing Practice (GMP) system to ensure stable and large-scale production of clinical-grade UDSCs from patient urine. In this study, we evaluated the in vivo safety and distribution of Klotho-enhanced UDSCs after intravenous administration in accordance with Good L

SUBMITTER: Kim SH 

PROVIDER: S-EPMC10712141 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature