Development of a contacting transwell co-culture system for the in vitro propagation of primary central nervous system lymphoma.

ABSTRACT: Primary central nervous system lymphoma (PCNSL) is a malignant neoplasm of the central nervous system that is refractory to treatment and has extremely poor prognosis. One factor hindering the development of therapeutic options for PCNSL is its molecular heterogeneity and the extreme difficulty in establishing in vitro cell lines that permit intensive research on this disease. In the present study, we developed a method to propagate PCNSL cells in vitro using a contacting transwell cell culture system involving brain vascular pericytes. The co-culture system was found to recapitulate the tumor microenvironment that is influenced by the biological activity of adjacent pericytes, and to sustain the survival and proliferation of PCNSL cells in vitro. We further delineated

SUBMITTER: Nishi M 

PROVIDER: S-EPMC10712316 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature