Rapid Identification of Seven Common ABO Alleles by Two-Dimensional Polymerase Chain Reaction Technology.

ABSTRACT:

Introduction

The molecular biology detection technology of the human ABO blood group system makes up for the limitations in many aspects compared with conventional serological typing technology. This study aimed to establish a new method to identify seven common ABO alleles (ABO*A1.01, ABO*A1.02, ABO*A2.01, ABO*B.01, ABO*O.01.01, ABO*O.01.02, and ABO*O.02.01) by two-dimensional polymerase chain reaction (2D PCR). 2D PCR can identify multiple target genes in a closed test tube by labeling specific primers with tags homologous to the sequence of fluorescently labeled probes, and melting curve analysis is performed after the fluorescent probes are hybridized with tag complem

SUBMITTER: Chen J 

PROVIDER: S-EPMC10712971 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature