NMJ Analyser: a novel method to quantify neuromuscular junction morphology in zebrafish.

ABSTRACT:

Motivation

Neuromuscular junction (NMJ) structural integrity is crucial for transducing motor neuron signals that initiate skeletal muscle contraction. Zebrafish has emerged as a simple and efficient model to study NMJ structural morphology and function in the context of developmental neurobiology and neuromuscular diseases. However, methods to quantify NMJ morphology from voluminous data of NMJ confocal images accurately, rapidly, and reproducibly are lacking.

Results

We developed an ImageJ macro called "NMJ Analyser" to automatically and unbiasedly analyse NMJ morphology in zebrafish. From the Z-stack of a zebrafish hemisomite, both presynaptic and postsynaptic fluorescently labeled termini at NMJs are extracted from background signal, with larger clusters of termini being

SUBMITTER: Singh J 

PROVIDER: S-EPMC10713120 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature