Identification of topoisomerase 2A as a novel bone metastasis-related gene in liver hepatocellular carcinoma.

ABSTRACT:

Background

Bone is the second most frequent site of metastasis for Liver hepatocellular carcinoma (LIHC), which leads to an extremely poor prognosis. Identifying novel biomarkers and therapeutic targets for LIHC patients with bone metastasis is urgently needed.

Methods

In this study, we used multiple databases for comprehensive bioinformatics analysis, including TCGA, GEO, ICGC, GTEx, TISIDB, and TIMER, to identify key genes related to bone metastasis of LIHC. Clinical tissues and tissue microarray were adopted to assess the expression of TOP2A through qRT-PCR and immunohistochemistry analyses in LIHC. Gene enrichment analysis, DNA methylation, gene mutation, prognosis, and tumor immunity associated with TOP2A in LIHC were investigated. In vitro and in vivo exp

SUBMITTER: Feng J 

PROVIDER: S-EPMC10713393 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature