Multimodal spatiotemporal phenotyping of human retinal organoid development.

ABSTRACT: Organoids generated from human pluripotent stem cells provide experimental systems to study development and disease, but quantitative measurements across different spatial scales and molecular modalities are lacking. In this study, we generated multiplexed protein maps over a retinal organoid time course and primary adult human retinal tissue. We developed a toolkit to visualize progenitor and neuron location, the spatial arrangements of extracellular and subcellular components and global patterning in each organoid and primary tissue. In addition, we generated a single-cell transcriptome and chromatin accessibility timecourse dataset and inferred a gene regulatory network underlying organoid development. We integrated genomic data with spatially segmented nuclei into a multimodal atlas to

SUBMITTER: Wahle P 

PROVIDER: S-EPMC10713453 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature