Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease.

ABSTRACT: The epicardium, the mesothelial envelope of the vertebrate heart, is the source of multiple cardiac cell lineages during embryonic development and provides signals that are essential to myocardial growth and repair. Here we generate self-organizing human pluripotent stem cell-derived epicardioids that display retinoic acid-dependent morphological, molecular and functional patterning of the epicardium and myocardium typical of the left ventricular wall. By combining lineage tracing, single-cell transcriptomics and chromatin accessibility profiling, we describe the specification and differentiation process of different cell lineages in epicardioids and draw comparisons to human fetal development at the transcriptional and morphological levels. We then use epicardioids to investigate the func

SUBMITTER: Meier AB 

PROVIDER: S-EPMC10713454 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature