Single-cell transcriptome sequencing reveals spatial distribution of IL34⁺ cancer-associated fibroblasts in hepatocellular carcinoma tumor microenvironment.

ABSTRACT: We utilized scRNA-seq, a well-established technology, to uncover the gene expression characteristics of IL34⁺ CAFs within HCC. We analyzed the related mechanisms through in vitro and in vivo assays. To begin, we acquired scRNA-seq datasets about HCC, which enabled us to identify distinct cell subpopulations within HCC tissues. We conducted a differential analysis to pinpoint DEGs associated with normal fibroblasts (NFs) and CAFs. Subsequently, we isolated NFs and CAFs, followed by the sorting of IL34⁺ CAFs. These IL34⁺ CAFs were then co-cultured with T cells and HCC cells to investigate their potential role in Tregs infiltration, CD8⁺ T cell toxicity, and the biological processes of HCC cells. We validated our findings in vivo using a well-establ

SUBMITTER: Wang G 

PROVIDER: S-EPMC10713639 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature