USP7 promotes non-small-cell lung cancer cell glycolysis and survival by stabilizing and activating c-Abl.

ABSTRACT:

Background

Abelson tyrosine kinase (c-Abl) is frequently mutated and highly expressed, and promotes non-small-cell lung cancer (NSCLC) survival, metastasis and tumorigenesis. c-Abl could also be modified through ubiquitination, but the underlying mechanism is not well understood.

Methods

Mass spectrometry assays were performed to search c-Abl deubiquitination enzymes. The molecular mechanism was determined using Co-IP assays, pull-down assays, Western blotting upon gene knockdown or overexpression. Cell lines and animal models were used to investigate the role of c-Abl and USP7 in NSCLC. EdU staining assay and Transwell assay were performed to evaluate the proliferation and migration ability of NSCLC cells, respectively.

Results

Ubiquitin-specific protease 7 (USP7) is

SUBMITTER: He Y 

PROVIDER: S-EPMC10713873 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature