Amplicon deep sequencing of five highly polymorphic markers of Plasmodium falciparum reveals high parasite genetic diversity and moderate population structure in Ethiopia.

ABSTRACT:

Background

Plasmodium falciparum genetic diversity can add information on transmission intensity and can be used to track control and elimination interventions.

Methods

Dried blood spots (DBS) were collected from patients who were recruited for a P. falciparum malaria therapeutic efficacy trial in three malaria endemic sites in Ethiopia from October to December 2015, and November to December 2019. qPCR-confirmed infections were subject to amplicon sequencing of polymorphic markers ama1-D3, csp, cpp, cpmp, msp7. Genetic diversity, the proportion of multiclonal infections, multiplicity of infection, and population structure were analysed.

Results

Among 198 samples selected for sequencing, data was obtained for 181 samples. Mean MOI was 1.38 (95% CI 1.24-1.53) and 17% (3

SUBMITTER: Reda AG 

PROVIDER: S-EPMC10714478 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature