Comparison of phage-derived recombinases for genetic manipulation of Pseudomonas species.

ABSTRACT:

Importance

The Pseudomonas genus contains many members currently being investigated for applications in biodegradation, biopesticides, biocontrol, and synthetic biology. Though several strains have been identified with beneficial properties, chromosomal manipulations to further improve these strains for commercial applications have been limited due to the lack of efficient genetic tools that have been tested across this genus. Here, we test the recombineering efficiencies of five phage-derived recombinases across three biotechnologically relevant Pseudomonas strains: P. putida KT2440, P. protegens Pf-5, and P. protegens CHA0. These results demonstrate a method to generate targeted mutations quickly and efficiently across these strains, ideally int

SUBMITTER: Kalb MJ 

PROVIDER: S-EPMC10714826 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature