A novel, highly sensitive, one-tube nested quantitative real-time PCR for <i>Brucella</i> in human blood samples.

ABSTRACT:

Importance

This study developed a highly sensitive and efficient method for the detection of brucellosis by introducing a one-tube nested quantitative real-time PCR (qPCR) approach, representing a remarkable advance in the field. The method demonstrated an impressive analytical sensitivity of 100 fg/μL, surpassing conventional qPCR and enabling the detection of even low levels of Brucella DNA. In addition, the study's comprehensive evaluation of 250 clinical samples revealed a specificity of 100% and a sensitivity of 98.6%, underscoring its reliability and accuracy. Most importantly, the new method significantly improved the detection rate of low-burden samples, reducing cycle threshold values by an average of 6.4. These results underscore the immense potential of this appro

SUBMITTER: Liu L 

PROVIDER: S-EPMC10714840 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature