Prion Protein Overexpression in Adipose-Derived Mesenchymal Stem Cells (ADMSCs) Effectively Protected Rodent Kidney Against Ischemia-Reperfusion Injury Via Enhancing ATP/Mitochondrial Biogenesis-Role of ADMSC Rejuvenation and Proliferation.

ABSTRACT:

Background

We tested the hypothesis that overexpression of cellular-prion-protein in adipose-derived mesenchymal stem cells (PrPC^OE-ADMSCs) effectively protected the kidney against ischemia-reperfusion (IR) injury in rat.

Methods

Part I of cell culture was categorized into A1(ADMSCs)/A2(ADMSCs+p-Cresol)/A3(PrPC^OE in ADMSCs)/A4 (PrPC^OE in ADMSCs+p-Cresol). Part II of cell culture was divided into B1(ADMSCs)/B2[ADMSCs+lipopolysaccharide (LPS)]/B3(PrPC^OE in ADMSCs)/B4(PrPC^OE in ADMSCs+LPS). Sprague-Dawley (SD) rats (n = 50) were equally categorized into groups 1 (sham-operated-control)/2 (IR)/3 (IR+ADMSCs/6.0 × 10⁵ equally divided into bilateral-renal arteries and 6.0 × 10⁵ intravenous administration b

SUBMITTER: Chen YT 

PROVIDER: S-EPMC10714882 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature