Molecular diagnosis of visceral leishmaniasis: a French study comparing a reference PCR method targeting kinetoplast DNA and a commercial kit targeting ribosomal DNA.

ABSTRACT: Visceral leishmaniasis is a life-threatening disease caused by the Leishmania donovani species complex. For direct diagnosis, molecular diagnosis on blood and bone marrow is an increasingly used technique. For Leishmania, most PCR assays are laboratory-developed, but marketed PCR assays are now available and should be evaluated independently of manufacturers. The "quanty Leishmaniae, Clonit" kit was compared to a laboratory-developed method widely used in France and considered here as a reference method. Performances were evaluated on serial dilution assays, on 5 external quality controls and on 35 clinical samples. The reference method performed better than the Clonit kit with higher "performance scores": 20 of 28 (71%) and 4 of 12 (33%) vs 17 of 28 (61%) and 1 of 12 (8%) fo

SUBMITTER: Pasquier G 

PROVIDER: S-EPMC10714885 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature