Leptospira transcriptome sequencing using long-read technology reveals unannotated transcripts and potential polyadenylation of RNA molecules.

ABSTRACT: Leptospirosis, caused by the spirochete bacteria Leptospira, is an emerging zoonosis that causes life-threatening disease in humans and animals. However, a comprehensive understanding of leptospiral RNA profiles is limited. In this study, we sequenced and analyzed the transcriptome of multiple Leptospira strains using Oxford Nanopore Technologies' direct cDNA and direct RNA sequencing methods. We identified new operons, RNA molecules, and evidence of potential posttranscriptional polyadenylation in Leptospira transcriptomes. Some RNA molecules that had not been previously annotated could be potential sRNA or noncoding RNA molecules that play a role in gene expression regulation in Leptospira. Interestingly, the majority of the new RNA molecules identified in thi

SUBMITTER: Xu R 

PROVIDER: S-EPMC10715090 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature