DNA-methylation signature accurately differentiates pancreatic cancer from chronic pancreatitis in tissue and plasma.

ABSTRACT:

Objective

Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy. Differentiation from chronic pancreatitis (CP) is currently inaccurate in about one-third of cases. Misdiagnoses in both directions, however, have severe consequences for patients. We set out to identify molecular markers for a clear distinction between PDAC and CP.

Design

Genome-wide variations of DNA-methylation, messenger RNA and microRNA level as well as combinations thereof were analysed in 345 tissue samples for marker identification. To improve diagnostic performance, we established a random-forest machine-learning approach. Results were validated on another 48 samples and further corroborated in 16 liquid biopsy samples.

Results

Machine-learning succeeded in defining markers to different

SUBMITTER: Wu Y 

PROVIDER: S-EPMC10715533 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature