Phosphorylation bar-coding of free fatty acid receptor 2 is generated in a tissue-specific manner.

ABSTRACT: Free fatty acid receptor 2 (FFAR2) is activated by short-chain fatty acids and expressed widely, including in white adipocytes and various immune and enteroendocrine cells. Using both wild-type human FFAR2 and a designer receptor exclusively activated by designer drug (DREADD) variant we explored the activation and phosphorylation profile of the receptor, both in heterologous cell lines and in tissues from transgenic knock-in mouse lines expressing either human FFAR2 or the FFAR2-DREADD. FFAR2 phospho-site-specific antisera targeting either pSer²⁹⁶/pSer²⁹⁷ or pThr³⁰⁶/pThr³¹⁰ provided sensitive biomarkers of both constitutive and agonist-mediated phosphorylation as well as an effective means to visualise agonist-activated receptors in situ. In whi

SUBMITTER: Barki N 

PROVIDER: S-EPMC10715726 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature