M⁶A governs length-dependent enrichment of mRNAs in stress granules.

ABSTRACT: Stress granules are biomolecular condensates composed of protein and mRNA. One feature of stress granule-enriched mRNAs is that they are often longer than average. Another feature of stress granule-enriched mRNAs is that they often contain multiple N⁶-methyladenosine (m⁶A) residues. m⁶A is bound by the YTHDF proteins, creating mRNA-protein complexes that partition into stress granules in mammalian cells. Here we show that length-dependent enrichment of mRNAs in stress granules is mediated by m⁶A. Long mRNAs often contain one or more long exons, which are preferential sites of m⁶A formation. In mammalian cells lacking m⁶A, long mRNAs no longer show preferential stress granule enrichment. Furthermore, we show that m⁶

SUBMITTER: Ries RJ 

PROVIDER: S-EPMC10715973 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature