Silencing of PD-1 combined with EBV-specific killer T cells for the treatment of EBV-associated B lymphoma.

ABSTRACT: Epstein-Barr Virus (EBV) infection is closely associated with the development of lymphoma, as it plays a significant role in the malignant transformation of lymphocytes. The expression of programmed death-1 (PD-1), which binds to PD-L1 in tumor cells, can lead to immune evasion by lymphoma cells and promote tumor progression. In this study, immortalized B lymphoblastoid cell lines (B-LCLs) positive for EBV-specific proteins were established from human peripheral mononuclear cells (PBMCs) using EBV induction along with CpG-ODN 2006 and cyclosporin A. EBV-specific T cells (EBVST) were generated by multiple immunizations of CD3⁺ T lymphocytes using irradiated B-LCLs. Flow cytometry analysis confirmed the activation of EBVST through the detection of CD3⁺, CD4⁺,

SUBMITTER: Wang J 

PROVIDER: S-EPMC10716022 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature