Deep Learning-Assisted Design of Novel Promoters in Escherichia coli.

ABSTRACT: Deep learning (DL) approaches have the ability to accurately recognize promoter regions and predict their strength. Here, the potential for controllably designing active Escherichia coli promoter is explored by combining multiple deep learning models. First, "DRSAdesign," which relies on a diffusion model to generate different types of novel promoters is created, followed by predicting whether they are real or fake and strength. Experimental validation showed that 45 out of 50 generated promoters are active with high diversity, but most promoters have relatively low activity. Next, "Ndesign," which relies on generating random sequences carrying functional -35 and -10 motifs of the sigma70 promoter is introduced, and their strength is predicted using the designed DL model. The DL mod

SUBMITTER: Wang X 

PROVIDER: S-EPMC10716054 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature