Impact of wet-lab protocols on quality of whole-genome short-read sequences from foodborne microbial pathogens.

ABSTRACT: For successful elucidation of a food-borne infection chain, the availability of high-quality sequencing data from suspected microbial contaminants is a prerequisite. Commonly, those investigations are a joint effort undertaken by different laboratories and institutes. To analyze the extent of variability introduced by differing wet-lab procedures on the quality of the sequence data we conducted an interlaboratory study, involving four bacterial pathogens, which account for the majority of food-related bacterial infections: Campylobacter spp., Shiga toxin-producing Escherichia coli, Listeria monocytogenes, and Salmonella enterica. The participants, ranging from German federal research institutes, federal state laboratories to universities and companies, were aske

SUBMITTER: Forth LF 

PROVIDER: S-EPMC10716212 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature