Clinical application of amplification-based versus amplification-free metagenomic next-generation sequencing test in infectious diseases.

ABSTRACT:

Background

Recently, metagenomic next-generation sequencing (mNGS) has been used in the diagnosis of infectious diseases (IDs) as an emerging and powerful tool. However, whether the complicated methodological variation in mNGS detections makes a difference in their clinical performance is still unknown. Here we conducted a method study on the clinical application of mNGS tests in the DNA detection of IDs.

Methods

We analyzed the effect of several potential factors in the whole process of mNGS for DNA detection on microorganism identification in 98 samples of suspected ID patients by amplification-based mNGS. The amplification-based and amplification-free mNGS tests were successfully performed in 41 samples. Then we compared the clinical application of the two mNGS methods in

SUBMITTER: Wang ZY 

PROVIDER: S-EPMC10716234 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature