Video-rate 3D imaging of living cells using Fourier view-channel-depth light field microscopy.

ABSTRACT: Interrogation of subcellular biological dynamics occurring in a living cell often requires noninvasive imaging of the fragile cell with high spatiotemporal resolution across all three dimensions. It thereby poses big challenges to modern fluorescence microscopy implementations because the limited photon budget in a live-cell imaging task makes the achievable performance of conventional microscopy approaches compromise between their spatial resolution, volumetric imaging speed, and phototoxicity. Here, we incorporate a two-stage view-channel-depth (VCD) deep-learning reconstruction strategy with a Fourier light-field microscope based on diffractive optical element to realize fast 3D super-resolution reconstructions of intracellular dynamics from single diffraction-limited 2D light-filed mea

SUBMITTER: Yi C 

PROVIDER: S-EPMC10716377 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature