Deciphering the age-dependent changes of pulmonary fibroblasts in mice by single-cell transcriptomics.

ABSTRACT: Background and objectives: The heterogeneity of pulmonary fibroblasts, a critical aspect of both murine and human models under physiological and pathological conditions, is well-documented. Yet, consensus remains elusive on the subtypes, lineage, biological attributes, signal transduction pathways, and plasticity of these fibroblasts. This ambiguity significantly impedes our understanding of the fibrotic processes that transpire in lung tissue during aging. This study aims to elucidate the transcriptional profiles, differentiation pathways, and potential roles of fibroblasts within aging pulmonary tissue. Methods: We employed single-cell transcriptomic sequencing via the 10x Genomics platform. The downstream data were processed and analyzed using R packages, including Seurat.

SUBMITTER: Wu R 

PROVIDER: S-EPMC10716426 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature