A patterned human primitive heart organoid model generated by pluripotent stem cell self-organization.

ABSTRACT: Pluripotent stem cell-derived organoids can recapitulate significant features of organ development in vitro. We hypothesized that creating human heart organoids by mimicking aspects of in utero gestation (e.g., addition of metabolic and hormonal factors) would lead to higher physiological and anatomical relevance. We find that heart organoids produced using this self-organization-driven developmental induction strategy are remarkably similar transcriptionally and morphologically to age-matched human embryonic hearts. We also show that they recapitulate several aspects of cardiac development, including large atrial and ventricular chambers, proepicardial organ formation, and retinoic acid-mediated anterior-posterior patterning, mimicking the developmental processes found in the post-heart t

SUBMITTER: Volmert B 

PROVIDER: S-EPMC10716495 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature