Detectability of and interference by major and minor hemoglobin variants using a new-generation ion-exchange HPLC system with two switchable analysis modes.

ABSTRACT:

Objectives

High-performance liquid chromatography (HPLC) is commonly used to measure hemoglobin A_1c (HbA_1c) levels and detect hemoglobin variants (Hb-Vars). HLC-723GR01 (GR01) is a new-generation automated ion-exchange HPLC system with two switchable analysis modes, namely short (30 s/test) and long modes (50 s/test). We evaluated the general performance of both analysis modes of GR01 for quantifying HbA_1c and detecting Hb-Vars.

Design and methods

We evaluated the instrument's precision based on CLSI protocol EP-05-A3. A comparison of the two analysis modes of GR01 against the standard mode of HLC-723G11 was performed on 100 whole blood samples. The GR01 long mode was compared with affinity HPLC (AF-HPLC) for detecting common Hb-Vars (HbE, Hb

SUBMITTER: Manita D 

PROVIDER: S-EPMC10716769 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature