A positive feedback circuit driven by m⁶A-modified circular RNA facilitates colorectal cancer liver metastasis.

ABSTRACT:

Background

Liver metastasis is the leading cause of death in patients with colorectal cancer (CRC). Emerge evidence suggests that circular RNA (circRNA) is a pivotal player in cancer progression. However, its role in CRC liver metastasis remains largely unknown.

Methods

Circ-YAP expression was detected by qRT-PCR and in situ hybridization. The function of circ-YAP was tested by wound healing, transwell and CCK-8 assays. RNA immunoprecipitation, pull-down, luciferase reporter, chromatin immunoprecipitation assays were used to investigate the mechanism underlying circ-YAP promoting CRC liver metastasis. CRC liver metastasis animal model was established to assess the effect of circ-YAP in vivo.

Results

Circ-YAP was notably upregulated in CRC with liver metastasis, which

SUBMITTER: Zeng K 

PROVIDER: S-EPMC10717141 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature