NK cell-derived exosomes improved lung injury in mouse model of Pseudomonas aeruginosa lung infection.

ABSTRACT:

Background

Pseudomonas aeruginosa (PA) is one of the most common bacteria that causes lung infection in hospital. The aim of our study is to explore the role and action mechanism of NK cells in lung PA infection.

Methods

In this present study, 2.5 × 10⁸ CFU/mouse PA was injected into murine trachea to make lung PA infection mouse model. Anti-asialo GM1 was used to inhibit NK cell. The percentage of NK cells was ensured by flow cytometry, and the M1- and M2-polarized macrophages were determined by flow cytometry, qRT-PCR, and ELISA assay. Besides, H&E staining was performed to ensure the pathological changes in lung tissues. Transmission electron microscopy and western blot were carried out to identify the exosome.

Results

Here, in the mouse model of PA lung

SUBMITTER: Jia R 

PROVIDER: S-EPMC10717361 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature