Molecular mechanism and therapeutic potential of HDAC9 in intervertebral disc degeneration.

ABSTRACT:

Background

Intervertebral disc degeneration (IVDD) is the major cause of low-back pain. Histone deacetylase 9 (HDAC9) was dramatically decreased in the degenerative nucleus pulposus (NP) samples of patients with intervertebral disc degeneration (IVDD) according to bioinformatics analysis of Gene Expression Omnibus (GEO) GSE56081 dataset. This study aims to investigate the role of HDAC9 in IVDD progression.

Methods

The contribution of HDAC9 to the progression of IVDD was assessed using HDAC9 knockout (HDAC9^KO) mice and NP-targeted HDAC9-overexpressing mice by IVD injection of adenovirus-mediated HDAC9 under a Col2a1 promoter. Magnetic resonance imaging (MRI) and histological analysis were used to examine the degeneration of IVD. NP cells were isolated from mice to

SUBMITTER: Lei M 

PROVIDER: S-EPMC10717711 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature