Native mass spectrometry of proteoliposomes containing integral and peripheral membrane proteins.

ABSTRACT: Cellular membranes are critical to the function of membrane proteins, whether they are associated (peripheral) or embedded (integral) within the bilayer. While detergents have contributed to our understanding of membrane protein structure and function, there remains challenges in characterizing protein-lipid interactions within the context of an intact membrane. Here, we developed a method to prepare proteoliposomes for native mass spectrometry (MS) studies. We first use native MS to detect the encapsulation of soluble proteins within liposomes. We then find the peripheral G_β1γ2 complex associated with the membrane can be ejected and analyzed using native MS. Four different integral membrane proteins (AmtB, AqpZ, TRAAK, and TREK2), all of which have previously been characterized

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC10718073 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature