IL-35 Stabilizes Treg Phenotype to Protect Cardiac Allografts in Mice.

ABSTRACT:

Background

Interleukin-35 (IL-35), secreted by regulatory T cells (Treg) and B cells, is immunosuppressive under both physiological and pathological conditions. However, the role of IL-35 in all responses has yet to be investigated. Here, we demonstrate that IL-35 protects allografts by stabilizing the Treg phenotype and suppressing CD8 + T-cell activation in a mouse heart transplantation model.

Methods

The effect of IL-35 on immune cell infiltration in grafts and secondary lymphoid organs was examined using mass cytometry, flow cytometry, and immunofluorescence. Moreover, using quantitative real-time polymerase chain reaction, flow cytometry, and phospho-flow assays, we demonstrated that IL-35 maintains Treg phenotypes to restrain CD8 + T cells via the gp130/signal transduc

SUBMITTER: Huang A 

PROVIDER: S-EPMC10718222 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature