Automated measurement of distance-walked as a "sixth vital sign" for detecting infusion reactions during preclinical testing.

ABSTRACT: Infusion reactions are a major risk for advanced therapeutics (e.g., engineered proteins nanoparticles, etc.), which can trigger the complement cascade, anaphylaxis, and other life-threatening immune responses. However, during the early phases of development, it is uncommon to assess for infusion reactions, given the labor involved in measuring multiple physiological parameters in rodents. Therefore, we sought to develop an automated quantification of rodent locomotion to serve as a sensitive screening tool for infusion reactions, with minimal added labor-time for each experiment. Here we present the detailed methods for building a motion tracking cage for mice, requiring ∼$100 of materials, ∼2 h to build and set up completely, and employing freely available software (DeepLabCut). The dist

SUBMITTER: Chase LS 

PROVIDER: S-EPMC10718583 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature