Compartment-driven imprinting of intestinal CD4 T cells in inflammatory bowel disease and homeostasis.

ABSTRACT: The mucosal immune system is implicated in the etiology and progression of inflammatory bowel diseases. The lamina propria and epithelium of the gut mucosa constitute two separate compartments, containing distinct T cell populations. Human CD4 T cell programming and regulation of lamina propria and epithelium CD4 T cells, especially during inflammation, remains incompletely understood. We performed flow cytometry, bulk and single-cell RNA-sequencing to profile ileal lamina propria and intraepithelial CD4 T cells (CD4CD8αα, regulatory T cells (Tregs), CD69 - and CD69 high Trm T cells) in controls and Crohn's disease (CD) patients (paired non-inflamed and inflamed). Inflammation results in alterations of the CD4 T cell population with a pronounced increase in Tregs and migrating/infiltrating

SUBMITTER: Lutter L 

PROVIDER: S-EPMC10719222 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature