Benchmarking DNA isolation methods for marine metagenomics.

ABSTRACT: Metagenomics is a powerful tool to study marine microbial communities. However, obtaining high-quality environmental DNA suitable for downstream sequencing applications is a challenging task. The quality and quantity of isolated DNA heavily depend on the choice of purification procedure and the type of sample. Selection of an appropriate DNA isolation method for a new type of material often entails a lengthy trial and error process. Further, each DNA purification approach introduces biases and thus affects the composition of the studied community. To account for these problems and biases, we systematically investigated efficiency of DNA purification from three types of samples (water, sea sediment, and digestive tract of a model invertebrate Magallana gigas) with eight commercially availab

SUBMITTER: Demkina A 

PROVIDER: S-EPMC10719357 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature