Protocols for sample preparation and compound-specific stable-isotope analyses (δ²H, δ¹³C) of fatty acids in biological and environmental samples.

ABSTRACT: Compound-specific stable-isotope analysis (CSIA) of fatty acids is a powerful tool to better understand the trophic transfer of fatty acids and their biochemical fate in and across ecosystems, including tracing animal migration and understanding physiological processes. The non-exchangeable nature of C-H bonds in acyl chains, hydrogen (δ²H) and carbon (δ¹³C) stable-isotope values of fatty acids (FA) provide independent information about the origins of fatty acids. Several technical obstacles must be overcome to ensure accurate and reproducible measurements of FA-CSIA can be made. This protocol describes the sample preparation process for successful stable-isotope analyses of fatty acids obtained from environmental and biological samples. Numerous techniq

SUBMITTER: Pilecky M 

PROVIDER: S-EPMC10719507 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature