Improved image processing for 3D virtual object construction from serial sections reveals tissue patterns in root tips of Zea mays.

ABSTRACT:

Premise

Previously we described methods for generating three-dimensional (3D) virtual reconstructions of plant tissues from transverse thin sections. Here, we report the applicability of longitudinal sections and improved image-processing steps that are simpler to perform and utilize free applications.

Methods

In order to obtain improved digital images and a virtual 3D object (cuboid), GIMP 2.10 and ImageJ 2.3.0 running on a laptop computer were used. Sectional views of the cuboid and 3D visualization were realized with use of the plug-ins "Volume Viewer" and "3D Viewer" in ImageJ.

Results

A 3D object was constructed and sectional views along several cutting planes were generated. The 3D object consisted of selected tissues inside the cuboid that were extracted and vi

SUBMITTER: Miki Y 

PROVIDER: S-EPMC10719874 | biostudies-literature | 2023 Nov-Dec

REPOSITORIES: biostudies-literature